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常用荧光染料谱

   日期:2015-05-03     浏览:154    评论:0    
核心提示:染料名称Excitation(nm)Emission(nm)分子量备注信息Reactive and conjugated probesHydroxycoumarin325386331Succinimidyl esterAminocoumarin350445330Succinimidyl esterMethoxycoumarin360410317Succinimidyl esterCascade Blue(375);401423596HydrazidePacific Blue403455406Maleimi
染料名称
Excitation(nm)
Emission(nm)
分子量
备注信息
Reactive and conjugated probes
Hydroxycoumarin
325
386
331
Succinimidyl ester
Aminocoumarin
350
445
330
Succinimidyl ester
Methoxycoumarin
360
410
317
Succinimidyl ester
Cascade Blue
(375);401
423
596
Hydrazide
Pacific Blue
403
455
406
Maleimide
Pacific Orange
403
551
   
Lucifer yellow
425
528
   
NBD
466
539
294
NBD-X
R-Phycoerythrin (PE)
480;565
578
240 k
 
PE-Cy5 conjugates
480;565;650
670
 
aka Cychrome, R670, Tri-Color, Quantum Red
PE-Cy7 conjugates
480;565;743
767
   
Red 613
480;565
613
 
PE-Texas Red
PerCP
490
675
 
Peridinin chlorphyll protein
TruRed
490,675
695
 
PerCP-Cy5.5 conjugate
FluorX
494
520
587
GE Healthcare
Fluorescein
495
519
389
FITC; pH sensitive
BODIPY-FL
503
512
   
TRITC
547
572
444
TRITC
X-Rhodamine
570
576
548
XRITC
Lissamine Rhodamine B
570
590
   
Texas Red
589
615
625
Sulfonyl chloride
Allophycocyanin (APC)
650
660
104 k
 
APC-Cy7 conjugates
650;755
767
 
PharRed
Alexa Fluor系列荧光染料
Alexa Fluor 350
343
442
410
 
Alexa Fluor 405
401
421
1028
 
Alexa Fluor 430
434
540
702
 
Alexa Fluor 488
499
519
643
QY 0.92
Alexa Fluor 500
503
525
700
 
Alexa Fluor 514
517
542
714
 
Alexa Fluor 532
530
555
724
QY 0.61
Alexa Fluor 546
561
572
1079
QY 0.79
Alexa Fluor 555
553
568
1250
QY 0.1
Alexa Fluor 568
579
603
792
QY 0.69
Alexa Fluor 594
591
618
820
QY 0.66
Alexa Fluor 610
610
629
1285
 
Alexa Fluor 633
632
648
1200
 
Alexa Fluor 647
652
668
1300
QY 0.33
Alexa Fluor 660
663
691
1100
 
Alexa Fluor 680
680
702
1150
 
Alexa Fluor 700
696
719
1400
 
Alexa Fluor 750
752
776
1300
 
Alexa Fluor 790
782
804
1750
 
Cy系列荧光染料
Cy2
489
506
714
QY 0.12
Cy3
(512);550
570;(615)
767
QY 0.15
Cy3B
558
572;(620)
658
QY 0.67
Cy3.5
581
594;(640)
1102
QY 0.15
Cy5
(625);650
670
792
QY 0.28
Cy5.5
675
694
1128
QY 0.23
Cy7
743
767
818
QY 0.28
核酸相关探针染料
Hoechst 33342
343
483
616
AT-selective
DAPI
345
455
 
AT-selective
Hoechst 33258
345
478
624
AT-selective
SYTOX Blue
431
480
~400
DNA
Chromomycin A3
445
575
 
CG-selective
Mithramycin
445
575
   
YOYO-1
491
509
1271
 
Ethidium Bromide
493
620
394
 
Acridine Orange
503
530/640
 
DNA/RNA
SYTOX Green
504
523
~600
DNA
TOTO-1, TO-PRO-1
509
533
 
Vital stain, TOTO: Cyanine Dimer
TO-PRO: Cyanine Monomer
Thiazole Orange
510
530
   
Propidium Iodide (PI)
536
617
668.4
 
LDS 751
543;590
712;607
472
DNA (543ex/712em), RNA (590ex/607em)
7-AAD
546
647
 
7-aminoactinomycin D, CG-selective
SYTOX Orange
547
570
~500
DNA
TOTO-3, TO-PRO-3
642
661
   
DRAQ5
647
681,697
413
usable excitation down to 488
细胞功能探针
Indo-1
361/330
490/405
1010
AM ester. Low/High Ca++,
Fluo-3
506
526
855
AM ester. pH > 6
DCFH
505
535
529
2'7'Dichorodihydrofluorescein, oxidized form
DHR
505
534
346
Dihydrorhodamine 123, oxidized form,
light catalyzes oxidation
SNARF
548/579
587/635
 
pH 6/9
荧光蛋白
       
QY
BR
PS
 
Y66H
360
442
         
Y66F
360
508
         
EBFP
380
440
 
0.18
0.27
 
monomer
EBFP2
383
448
   
20
 
monomer
Azurite
383
447
   
15
 
monomer
GFPuv
385
508
         
T-Sapphire
399
511
 
0.60
26
25
weak dimer
Cerulean
433
475
 
0.62
27
36
weak dimer
mCFP
433
475
 
0.40
13
64
monomer
ECFP
434
477
 
0.15
3
   
CyPet
435
477
 
0.51
18
59
weak dimer
Y66W
436
485
         
mKeima-Red
440
620
 
0.24
3
 
monomer
TagCFP
458
480
   
29
 
dimer
AmCyan1
458
489
 
0.75
29
 
tetramer
mTFP1
462
492
   
54
 
dimer
S65A
471
504
         
Midoriishi Cyan
472
495
 
0.9
25
 
dimer
Wild Type GFP
396,475
508
26k
0.77
     
S65C
479
507
         
TurboGFP
482
502
26 k
0.53
37
 
dimer
TagGFP
482
505
   
34
 
monomer
S65L
484
510
         
Emerald
487
509
 
0.68
39
0.69
weak dimer; (Invitrogen)
S65T
488
511
         
EGFP
488
507
26k
0.60
34
174
weak dimer
Azami Green
492
505
 
0.74
41
 
monomer
ZsGreen1
493
505
105k
0.91
40
 
tetramer
TagYFP
508
524
   
47
 
monomer
EYFP
514
527
26k
0.61
51
60
weak dimer
Topaz
514
527
   
57
 
monomer
Venus
515
528
 
0.57
53
15
weak dimer
mCitrine
516
529
 
0.76
59
49
monomer
YPet
517
530
 
0.77
80
49
weak dimer
TurboYFP
525
538
26 k
0.53
1.65
 
dimer
ZsYellow1
529
539
 
0.65
13
 
tetramer
Kusabira Orange
548
559
 
0.60
31
 
monomer
mOrange
548
562
 
0.69
49
9
monomer
mKO
548
559
 
0.60
31
122
monomer
TurboRFP
553
574
26 k
0.67
62
 
dimer
tdTomato
554
581
 
0.69
95
98
tandem dimer
TagRFP
555
584
   
50
 
monomer
DsRed monomer
556
586
~28k
0.1
3.5
16
monomer
DsRed2 ("RFP")
563
582
~110k
0.55
24
   
mStrawberry
574
596
 
0.29
26
15
monomer
TurboFP602
574
602
26 k
0.35
26
 
dimer
AsRed2
576
592
~110k
0.21
13
 
tetramer
mRFP1
584
607
~30k
0.25
   
monomer
J-Red
584
610
 
0.20
8.8
13
dimer
mCherry
587
610
 
0.22
16
96
monomer
HcRed1
588
618
~52k
0.03
0.6
 
dimer
Katusha
588
635
   
23
 
dimer
mKate (TagFP635)
588
635
   
15
 
monomer
TurboFP635
588
635
26 k
0.34
22
 
dimer
mPlum
590
649
 
0.10
4.1
53
 
mRaspberry
598
625
 
0.15
13
 
monomer; faster photobleach than mPlum
其他探针
Monochlorobimane
380
461
226
Glutathione probe
Calcein
496
517
623
pH > 5
 
 
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