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Application Dilution
ELISA 1:2000
ICC 1:50
IP 1:100
WB 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Kommaddi, R.P. et al. (2015) J Biol Chem. 3;290(14):8888 WB: COS-7 cellsTsai, C.F. et al. (2015) J Agric Food Chem. 9;63(48):10388 WB: Huh7 cellsKim, S. et al. (2013) Diabetes. 62(2):471. WB, IP: mouse adipocytesSroyraya, M. et al. (2013) Microsc Res Tech. 76(1):103. WB: Swimming CrabHummel, S. et al. (2012) Appl Environ Microbiol. 78(4):1140. WB: human colonic adenocarcinoma cellsLaluk, K. et al. (2011) Plant Cell 23(8):2831. WB: Arabidopsis BIK1 and Myelin basis proteinimageBackgroundPhosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.
Background
References
Hunter T.(1987) Cell. 50(6):823.Krishna, R.G. & Wold, F. (1993) Adv Enzymol Rel Areas Mol Biol 67:265.Yaffe, M.B. & Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.ImmunogenAnti-Phosphoserine/threonine was generated from a panel of phosphoserine and phosphothreonine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved in many species.Buffer and StorageRabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.SpecificityThis antibody was cross-adsorbed to unphosphorylated peptide then affinity purified using a mix of phosphoserine and phosphothreonine peptides (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.