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Actin Filament Regulation Immunocytochemistry Kit

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品牌: ECMBio
货号: 22-AK7600
规格: kit
应用: ICC, IHC
单价: 询价
所在地: 北京
有效期至: 长期有效
最后更新: 2016-12-22 16:49
询价
 
公司基本资料信息
详细说明
Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.

Kit Summary

The actin filament regualtion kit can be used to compare labeling between actin filaments and total actin. The kit contains two antibodies for detecting total actin, and Phalloidin:FITC for labeling actin filaments. In addition, secondary reagents conjugated to DyLight® 594 are included for dual labeling experiments with Phalloidin:FITC.

http://www.ecmbiosciences.com/index.php?content=TDS&catnum=AK7600
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