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培养基NCTC-109配方,不含谷氨酰胺

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NCTC-109 medium is a chemically defined medium that was originally developed by the National Cancer Institute and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment.

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 13.6 0.18133333
Hydroxy L-proline 131.0 4.09 0.031221375
L-Alanine 89.0 31.6 0.3550562
L-Arginine hydrochloride 211.0 31.16 0.14767772
L-Asparagine-H2O 150.0 9.17 0.061133333
L-Aspartic acid 133.0 9.91 0.074511275
L-Cysteine hydrochloride-H2O 176.0 290.0 1.6477273
L-Cystine 2HCl 313.0 14.0 0.044728436
L-Glutamic Acid 147.0 8.26 0.05619048
L-Histidine hydrochloride-H2O 210.0 26.65 0.12690476
L-Isoleucine 131.0 18.04 0.13770993
L-Leucine 131.0 20.44 0.15603054
L-Lysine hydrochloride 183.0 38.43 0.21000001
L-Methionine 149.0 4.44 0.029798659
L-Ornithine HCl 169.0 9.41 0.055680472
L-Phenylalanine 165.0 16.53 0.100181825
L-Proline 115.0 6.13 0.05330435
L-Serine 105.0 10.75 0.102380954
L-Taurine 125.0 4.18 0.033439998
L-Threonine 119.0 18.93 0.15907563
L-Tryptophan 204.0 17.5 0.085784316
L-Tyrosine disodium salt dihydrate 181.0 21.0 0.1160221
L-Valine 117.0 25.0 0.21367522
Vitamins
Biotin 244.0 0.025 1.0245902E-4
Choline chloride 140.0 1.25 0.008928572
D-Calcium pantothenate 477.0 0.025 5.2410902E-5
Folic Acid 441.0 0.025 5.6689343E-5
Menadione (Vitamin K3) 172.0 0.025 1.4534884E-4
Niacinamide 122.0 0.0625 5.1229505E-4
Nicotinic acid (Niacin) 123.0 0.0625 5.0813006E-4
Para-Aminobenzoic Acid 137.0 0.125 9.1240875E-4
Pyridoxal hydrochloride 204.0 0.0625 3.0637256E-4
Pyridoxine hydrochloride 206.0 0.0625 3.0339806E-4
Riboflavin 376.0 0.025 6.648936E-5
Thiamine hydrochloride 337.0 0.025 7.4183976E-5
Vitamin A (acetate) 286.0 0.25 8.7412586E-4
Vitamin B12 1355.0 10.0 0.007380074
Vitamin D2 (Calciferol) 397.0 0.25 6.297229E-4
alpha Tocopherol phos. Na salt 554.7 0.025 4.5069406E-5
i-Inositol 180.0 0.125 6.9444446E-4
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 200.0 1.8018018
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 97.67 0.8139166
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 6800.0 117.24138
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 140.0 1.0144928
Ribonucleosides
5-Methylcytosine 225.0 0.1 4.4444445E-4
Deoxyribonucleosides
2'Deoxyadenosine 251.0 10.0 0.03984064
2'Deoxycytidine HCl 264.0 10.0 0.03787879
2'Deoxyguanosine 267.0 10.0 0.037453182
Thymidine 242.0 10.0 0.041322313
Coenzymes
CoA (Coenzyme A) 768.0 2.5 0.0032552083
DPN (diphosphopyridine nucleotide) 663.0 7.0 0.01055807
FAD (flavin adenine dinucleotide) 786.0 1.0 0.0012722646
TPN (triphosphopyridine nucleotide-Na) 743.0 1.0 0.001345895
TPP Co-carboxylase 461.0 1.0 0.0021691974
UTP (uridine triphosphate 3Na-2H20) 586.0 1.0 0.0017064846
Reducing Agents
Ascorbic Acid 176.0 50.0 0.2840909
Glutathione, monosodium 329.0 10.0 0.030395137
Other Components
D-Glucosamine HCl 216.0 3.85 0.017824074
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
D-Glucuronolactone 176.0 1.8 0.010227272
L-alpha-Amino-n-butyric acid 103.0 5.5 0.053398058
Phenol Red 376.4 20.0 0.053134963
Sodium Acetate 136.0 50.0 0.36764705
Sodium glucuronate-H2O 236.0 1.8 0.0076271184
Tween 80®   12.5 Infinity

 
 
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